| dc.description.abstract | Objective: To evaluate the acute and sub-acute oral toxicity of
Cucumis metuliferus and Lippia kituiensis leaf chloroform extracts in
mice and rats model. Methods: Acute oral toxicity study of chloroform
extracts of L. kituiensis and C. metuliferus was carried out by
administration of 300, 600, 1500, mg/kg body weight to mice in the
respective groups. The LD50 of the C. metuliferus and L. kituiensis
extracts was determined to be not greater than 2000 mg/kg body
weight. Sub-acute toxicity study was conducted by oral administration
of the extracts at daily doses of 150, 300 and 500mg/kg body weight in
the respective groups of rats for 28 days, and a positive control
consisting of 1% DMSO and 5 mL was given to each. Results: In
acute toxicity, all treated groups revealed neither mortality nor
significant alteration in behavior, body weight, and hematology parameters. However, the
significance different was observed in organ weight at a dose of 600mg/kg and 1500mg/kg of
the tested plant extract in both plants. In sub acute study the result revealed neither mortality
nor significant alteration in behavior between treated and control. The significant different
was observed in body weight in all doses in both plants and organ weight and haematological
parameter in dose level 300 mg/kg and 500 mg/kg compared to control. Moreover the
significant change was observed in biochemical parameters of both sexes in dose of 300
mg/kg and 500 mg/kg body weight of C. metuliferus and L. kituiensis extract. A significant
histological change was observed in liver, kidney, lungs and spleen in all extract of C.
metuliferus and L. kituiensis in a dose of 300 mg/kg and 500 mg/kg body weight. Conclusion:
These plants can causes‟ severe toxicity to animals. | en_US |
